An ectopic study of apatite-coated silk fibroin scaffolds seeded with AdBMP-2 modified canine bMSCs
Identifieur interne : 004308 ( Main/Exploration ); précédent : 004307; suivant : 004309An ectopic study of apatite-coated silk fibroin scaffolds seeded with AdBMP-2 modified canine bMSCs
Auteurs : Kaige Lü [République populaire de Chine] ; Ling Xu [République populaire de Chine] ; Lunguo Xia [République populaire de Chine] ; Yilin Zhang [République populaire de Chine] ; Xiuli Zhang [République populaire de Chine] ; David L. Kaplan [États-Unis] ; Xinquan Jiang [République populaire de Chine] ; Fuqiang Zhang [République populaire de Chine]Source :
- Journal of Biomaterials Science. Polymer Edition [ 0920-5063 ] ; 2011.
Abstract
The present study was undertaken to evaluate ectopic new bone formation effects of apatite-coated silk fibroin scaffolds (mSS) seeded with adenovirus mediated bone morphogenic protein-2 gene (AdBMP-2) transduced canine bone marrow stromal cells (bMSCs) in nude mice. In this study, bMSCs derived from canine were cultured and transduced with AdBMP-2, adenovirus mediated enhanced green fluorescent protein gene (AdEGFP) in vitro. Osteogenic differentiation of bMSCs was determined by alkaline phosphatase (ALP) activity analysis, and the transcript levels for BMP-2, osteopontin (OPN), osteocalcin (OCN) and bone sialoprotein (BSP) genes via real-time quantitative PCR (RT-qPCR) analysis. The ectopic bone formation effects of mSS seeded with AdBMP-2 modified bMSCs were evaluated through histological and histomorphological analysis 4, 8 and 12 weeks post-operation in nude mice. ALP activity was statistically increased in AdBMP-2 group, when compared with control groups. The mRNA expression of BMP-2, OPN, OCN and BSP also statistically up-regulated 6, 9 days after AdBMP-2 transduction. Significantly higher bone volume was achieved in AdBMP-2-transduced bMSCs/mSS constructs than that of AdEGFP-transduced bMSCs/mSS or bMSCs/mSS groups at 4, 8 and 12 weeks (
Url:
DOI: 10.1163/092050610X552861
PubMed: 21294971
PubMed Central: 3406182
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 002754
- to stream Pmc, to step Curation: 002754
- to stream Pmc, to step Checkpoint: 002249
- to stream Ncbi, to step Merge: 003429
- to stream Ncbi, to step Curation: 003429
- to stream Ncbi, to step Checkpoint: 003429
- to stream Main, to step Merge: 004335
- to stream Main, to step Curation: 004308
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">An ectopic study of apatite-coated silk fibroin scaffolds seeded with AdBMP-2 modified canine bMSCs</title>
<author><name sortKey="Lu, Kaige" sort="Lu, Kaige" uniqKey="Lu K" first="Kaige" last="Lü">Kaige Lü</name>
<affiliation wicri:level="1"><nlm:aff id="A1">Department of Prosthodontics, College of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Key Laboratory of Stomatology Shanghai, 200011, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Prosthodontics, College of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Key Laboratory of Stomatology Shanghai, 200011</wicri:regionArea>
<wicri:noRegion>200011</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Xu, Ling" sort="Xu, Ling" uniqKey="Xu L" first="Ling" last="Xu">Ling Xu</name>
<affiliation wicri:level="1"><nlm:aff id="A1">Department of Prosthodontics, College of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Key Laboratory of Stomatology Shanghai, 200011, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Prosthodontics, College of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Key Laboratory of Stomatology Shanghai, 200011</wicri:regionArea>
<wicri:noRegion>200011</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Xia, Lunguo" sort="Xia, Lunguo" uniqKey="Xia L" first="Lunguo" last="Xia">Lunguo Xia</name>
<affiliation wicri:level="1"><nlm:aff id="A2">Department of Oral and Maxillofacial Surgery, College of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Oral and Maxillofacial Surgery, College of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011</wicri:regionArea>
<wicri:noRegion>Shanghai 200011</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zhang, Yilin" sort="Zhang, Yilin" uniqKey="Zhang Y" first="Yilin" last="Zhang">Yilin Zhang</name>
<affiliation wicri:level="1"><nlm:aff id="A1">Department of Prosthodontics, College of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Key Laboratory of Stomatology Shanghai, 200011, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Prosthodontics, College of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Key Laboratory of Stomatology Shanghai, 200011</wicri:regionArea>
<wicri:noRegion>200011</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zhang, Xiuli" sort="Zhang, Xiuli" uniqKey="Zhang X" first="Xiuli" last="Zhang">Xiuli Zhang</name>
<affiliation wicri:level="1"><nlm:aff id="A3">Shanghai Research Institute of Stomatology, Shanghai Key Laboratory of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Shanghai Research Institute of Stomatology, Shanghai Key Laboratory of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011</wicri:regionArea>
<wicri:noRegion>Shanghai 200011</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Kaplan, David L" sort="Kaplan, David L" uniqKey="Kaplan D" first="David L." last="Kaplan">David L. Kaplan</name>
<affiliation wicri:level="1"><nlm:aff id="A4">Department of Biomedical Engineering, Tufts University, Massachusetts 02155, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Biomedical Engineering, Tufts University, Massachusetts 02155</wicri:regionArea>
<wicri:noRegion>Massachusetts 02155</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Jiang, Xinquan" sort="Jiang, Xinquan" uniqKey="Jiang X" first="Xinquan" last="Jiang">Xinquan Jiang</name>
<affiliation wicri:level="1"><nlm:aff id="A1">Department of Prosthodontics, College of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Key Laboratory of Stomatology Shanghai, 200011, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Prosthodontics, College of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Key Laboratory of Stomatology Shanghai, 200011</wicri:regionArea>
<wicri:noRegion>200011</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="A3">Shanghai Research Institute of Stomatology, Shanghai Key Laboratory of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Shanghai Research Institute of Stomatology, Shanghai Key Laboratory of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011</wicri:regionArea>
<wicri:noRegion>Shanghai 200011</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zhang, Fuqiang" sort="Zhang, Fuqiang" uniqKey="Zhang F" first="Fuqiang" last="Zhang">Fuqiang Zhang</name>
<affiliation wicri:level="1"><nlm:aff id="A1">Department of Prosthodontics, College of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Key Laboratory of Stomatology Shanghai, 200011, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Prosthodontics, College of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Key Laboratory of Stomatology Shanghai, 200011</wicri:regionArea>
<wicri:noRegion>200011</wicri:noRegion>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">21294971</idno>
<idno type="pmc">3406182</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3406182</idno>
<idno type="RBID">PMC:3406182</idno>
<idno type="doi">10.1163/092050610X552861</idno>
<date when="2011">2011</date>
<idno type="wicri:Area/Pmc/Corpus">002754</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">002754</idno>
<idno type="wicri:Area/Pmc/Curation">002754</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">002754</idno>
<idno type="wicri:Area/Pmc/Checkpoint">002249</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">002249</idno>
<idno type="wicri:Area/Ncbi/Merge">003429</idno>
<idno type="wicri:Area/Ncbi/Curation">003429</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">003429</idno>
<idno type="wicri:doubleKey">0920-5063:2011:Lu K:an:ectopic:study</idno>
<idno type="wicri:Area/Main/Merge">004335</idno>
<idno type="wicri:Area/Main/Curation">004308</idno>
<idno type="wicri:Area/Main/Exploration">004308</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">An ectopic study of apatite-coated silk fibroin scaffolds seeded with AdBMP-2 modified canine bMSCs</title>
<author><name sortKey="Lu, Kaige" sort="Lu, Kaige" uniqKey="Lu K" first="Kaige" last="Lü">Kaige Lü</name>
<affiliation wicri:level="1"><nlm:aff id="A1">Department of Prosthodontics, College of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Key Laboratory of Stomatology Shanghai, 200011, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Prosthodontics, College of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Key Laboratory of Stomatology Shanghai, 200011</wicri:regionArea>
<wicri:noRegion>200011</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Xu, Ling" sort="Xu, Ling" uniqKey="Xu L" first="Ling" last="Xu">Ling Xu</name>
<affiliation wicri:level="1"><nlm:aff id="A1">Department of Prosthodontics, College of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Key Laboratory of Stomatology Shanghai, 200011, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Prosthodontics, College of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Key Laboratory of Stomatology Shanghai, 200011</wicri:regionArea>
<wicri:noRegion>200011</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Xia, Lunguo" sort="Xia, Lunguo" uniqKey="Xia L" first="Lunguo" last="Xia">Lunguo Xia</name>
<affiliation wicri:level="1"><nlm:aff id="A2">Department of Oral and Maxillofacial Surgery, College of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Oral and Maxillofacial Surgery, College of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011</wicri:regionArea>
<wicri:noRegion>Shanghai 200011</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zhang, Yilin" sort="Zhang, Yilin" uniqKey="Zhang Y" first="Yilin" last="Zhang">Yilin Zhang</name>
<affiliation wicri:level="1"><nlm:aff id="A1">Department of Prosthodontics, College of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Key Laboratory of Stomatology Shanghai, 200011, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Prosthodontics, College of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Key Laboratory of Stomatology Shanghai, 200011</wicri:regionArea>
<wicri:noRegion>200011</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zhang, Xiuli" sort="Zhang, Xiuli" uniqKey="Zhang X" first="Xiuli" last="Zhang">Xiuli Zhang</name>
<affiliation wicri:level="1"><nlm:aff id="A3">Shanghai Research Institute of Stomatology, Shanghai Key Laboratory of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Shanghai Research Institute of Stomatology, Shanghai Key Laboratory of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011</wicri:regionArea>
<wicri:noRegion>Shanghai 200011</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Kaplan, David L" sort="Kaplan, David L" uniqKey="Kaplan D" first="David L." last="Kaplan">David L. Kaplan</name>
<affiliation wicri:level="1"><nlm:aff id="A4">Department of Biomedical Engineering, Tufts University, Massachusetts 02155, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Biomedical Engineering, Tufts University, Massachusetts 02155</wicri:regionArea>
<wicri:noRegion>Massachusetts 02155</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Jiang, Xinquan" sort="Jiang, Xinquan" uniqKey="Jiang X" first="Xinquan" last="Jiang">Xinquan Jiang</name>
<affiliation wicri:level="1"><nlm:aff id="A1">Department of Prosthodontics, College of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Key Laboratory of Stomatology Shanghai, 200011, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Prosthodontics, College of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Key Laboratory of Stomatology Shanghai, 200011</wicri:regionArea>
<wicri:noRegion>200011</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="A3">Shanghai Research Institute of Stomatology, Shanghai Key Laboratory of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Shanghai Research Institute of Stomatology, Shanghai Key Laboratory of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011</wicri:regionArea>
<wicri:noRegion>Shanghai 200011</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zhang, Fuqiang" sort="Zhang, Fuqiang" uniqKey="Zhang F" first="Fuqiang" last="Zhang">Fuqiang Zhang</name>
<affiliation wicri:level="1"><nlm:aff id="A1">Department of Prosthodontics, College of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Key Laboratory of Stomatology Shanghai, 200011, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Prosthodontics, College of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Key Laboratory of Stomatology Shanghai, 200011</wicri:regionArea>
<wicri:noRegion>200011</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j">Journal of Biomaterials Science. Polymer Edition</title>
<idno type="ISSN">0920-5063</idno>
<idno type="eISSN">1568-5624</idno>
<imprint><date when="2011">2011</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p id="P1">The present study was undertaken to evaluate ectopic new bone formation effects of apatite-coated silk fibroin scaffolds (mSS) seeded with adenovirus mediated bone morphogenic protein-2 gene (AdBMP-2) transduced canine bone marrow stromal cells (bMSCs) in nude mice. In this study, bMSCs derived from canine were cultured and transduced with AdBMP-2, adenovirus mediated enhanced green fluorescent protein gene (AdEGFP) in vitro. Osteogenic differentiation of bMSCs was determined by alkaline phosphatase (ALP) activity analysis, and the transcript levels for BMP-2, osteopontin (OPN), osteocalcin (OCN) and bone sialoprotein (BSP) genes via real-time quantitative PCR (RT-qPCR) analysis. The ectopic bone formation effects of mSS seeded with AdBMP-2 modified bMSCs were evaluated through histological and histomorphological analysis 4, 8 and 12 weeks post-operation in nude mice. ALP activity was statistically increased in AdBMP-2 group, when compared with control groups. The mRNA expression of BMP-2, OPN, OCN and BSP also statistically up-regulated 6, 9 days after AdBMP-2 transduction. Significantly higher bone volume was achieved in AdBMP-2-transduced bMSCs/mSS constructs than that of AdEGFP-transduced bMSCs/mSS or bMSCs/mSS groups at 4, 8 and 12 weeks (<italic>P</italic>
<0.01). These results demonstrated that mSS seeded with AdBMP-2-transduced canine bMSCs can promote ectopic new bone formation and maturation in nude mice suggesting the potential of this silk scaffold based tissue engineered bone for further bone regeneration studies in canine models.</p>
</div>
</front>
</TEI>
<affiliations><list><country><li>République populaire de Chine</li>
<li>États-Unis</li>
</country>
</list>
<tree><country name="République populaire de Chine"><noRegion><name sortKey="Lu, Kaige" sort="Lu, Kaige" uniqKey="Lu K" first="Kaige" last="Lü">Kaige Lü</name>
</noRegion>
<name sortKey="Jiang, Xinquan" sort="Jiang, Xinquan" uniqKey="Jiang X" first="Xinquan" last="Jiang">Xinquan Jiang</name>
<name sortKey="Jiang, Xinquan" sort="Jiang, Xinquan" uniqKey="Jiang X" first="Xinquan" last="Jiang">Xinquan Jiang</name>
<name sortKey="Xia, Lunguo" sort="Xia, Lunguo" uniqKey="Xia L" first="Lunguo" last="Xia">Lunguo Xia</name>
<name sortKey="Xu, Ling" sort="Xu, Ling" uniqKey="Xu L" first="Ling" last="Xu">Ling Xu</name>
<name sortKey="Zhang, Fuqiang" sort="Zhang, Fuqiang" uniqKey="Zhang F" first="Fuqiang" last="Zhang">Fuqiang Zhang</name>
<name sortKey="Zhang, Xiuli" sort="Zhang, Xiuli" uniqKey="Zhang X" first="Xiuli" last="Zhang">Xiuli Zhang</name>
<name sortKey="Zhang, Yilin" sort="Zhang, Yilin" uniqKey="Zhang Y" first="Yilin" last="Zhang">Yilin Zhang</name>
</country>
<country name="États-Unis"><noRegion><name sortKey="Kaplan, David L" sort="Kaplan, David L" uniqKey="Kaplan D" first="David L." last="Kaplan">David L. Kaplan</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Santé/explor/EdenteV2/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 004308 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 004308 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Santé |area= EdenteV2 |flux= Main |étape= Exploration |type= RBID |clé= PMC:3406182 |texte= An ectopic study of apatite-coated silk fibroin scaffolds seeded with AdBMP-2 modified canine bMSCs }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:21294971" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \ | NlmPubMed2Wicri -a EdenteV2
This area was generated with Dilib version V0.6.32. |